Aspergillus in culture
Fungi of the Aspergillus genus are well known to most people as the lacy, fluffy mold that grows on foods kept a bit too long. There are over 180 species of Aspergillus fungi and they generally do not cause disease unless the host has a compromised immune system or gets a very big exposure to the fungus. The most common species to cause problems in veterinary patients are Aspergillus fumigatus and Aspergillus tereus.
Under the microscope Aspergillus looks like a beautiful flower but note the spores (called “conidia”) it sheds. These float in the air to colonize organic matter or infect a host when they land.
There are two common types of Aspergillus infection in the dog: Nasal (usually caused by Aspergillus fumigatus) and Systemic (usually caused by Aspergillus tereus)
To diagnose sinonasal aspergillosis two out of the following criteria must be met:
General anesthesia is necessary for imaging as well as for rhinoscopy. Most veterinary hospitals can manage nasal radiographs but rhinoscopy may require referral (though sometimes the yellow Aspergillus plaques can be seen with less specialized equipment).
The treatment of aspergillosis has been challenging for many years; fungal infections as a rule are slow to clear and for a long time the only available drugs had toxic side effects. Today, sinonasal aspergillosis has a good prognosis thanks to a unique treatment plan. The patient is anesthetized and the back of the throat and is closed off with gauze and inflatable balloon catheters called “foley catheters.” The nostrils are also closed off (the patient breathes through an endotracheal tube that is placed through the mouth) and a 1% solution of clotrimazole, a topical antifungal lotion, is infused into the nose and frontal sinuses. The solution incubates for an hour and the patient is periodically turned to be sure all the nooks and crannies of the sinuses are treated. At the end of the incubation, the clotrimazole is drained through the nostrils and suctioned out.
This treatment is highly effective, with an 86% success rate though about 1/3 of patients require several treatments. Most of the time the nasal discharge has resolved within a couple of weeks but if there is still evidence of continuing infection one month after the treatment, the patient should be rechecked and another treatment should be expected.
If there is evidence that the infection has eroded through the sinus bones and has penetrated the brain, the treatment described above cannot be used and oral medication is needed. Common medications used are itraconazole and fluconazole. Several months of therapy is needed and a 60-70% success rate has been reported.
As shown in the picture above, Aspergillus fungi shed microscopic spores which float in the air and which we inhale all the time. Fortunately, we have an assortment of safety mechanisms built into our bodies which prevent infection (sinuses to trap inhaled debris, ability to sneeze, mucus lining of the entire tract to trap debris, cilia to remove mucus with trapped debris, and an immune system to fight infection). If Aspergillus spores, called “conidia,” escape these mechanisms and begin to grow, they become more difficult for the body to remove. Some strains of fungi are more virulent than others or one may simply be exposed to a large number of spores. In disseminated aspergillosis, the fungus penetrates the respiratory tract and may travel to other organs via the bloodstream, creating a more serious fungal invasion. German shepherd dogs seem to be predisposed to this type of dissemination.
Symptoms experienced by the patient depend on where the fungus settles. Bone infection is common and the intervertebral discs of the spine also tend to be favorite spots for Aspergillus. This means that clinical features commonly include lameness, weakness, and incoordination. Frequently there are draining tracts (holes with pus or bloody discharge oozing out) in the areas of infection. Fever, weight loss, appetite loss, and uveitis (deep inflammation of the eye) are also features. Sadly, most dogs are already terminally ill by the time they are first examined.
Diagnosis is tricky. Bone destruction patterns on radiographs may be suggestive of fungal infection. If one is lucky, the organism can be identified in some of the draining fluid or in a tissue sample. If the organism is not visible, it may be cultured from fluid or tissue sample. Ideally, there would be a blood test for Aspergillus antibodies that could make the diagnosis non-invasively and, while such antibody tests exist, it is hard to interpret them as one needs to know what species of Aspergillus to test for.
Treatment is particularly frustrating for disseminated aspergillosis but some new drugs have been promising. Amphoterecin B is an older antifungal drug, largely replaced by newer drugs because of its high potential to cause kidney damage. The problem here is that the newer drugs, with the possible exception of long courses (several years) of itraconazole, are not particular effective in aspergillosis. To minimize the kidney effects, amphoterecin B can be encapsulated in liposomes (microscopic fatty envelopes) or other lipid formulations. These have been used in humans with some success but are extremely expensive. Terbinafine and voriconazole have been used in humans for disseminated aspergillosis but there is very little study of their use in pets.
Page last updated 1/29/09